The Beehive is located before security on a new mezzanine level overlooking the arrivals lounge where both passengers entering the UK and friends and relatives picking up passengers can access.
The pub joins restaurant brands Giraffe and Apostrophe which opened at the terminal earlier this year.
Guy Stephenson, commercial director for Gatwick, said: "We are always looking for new ways to expand and improve the range of food and drink establishments available to passengers, making sure that they have something to suit every mood and need.
“We now offer 'food on the go' outlets, more relaxed coffee houses as well as good quality sit down dining establishments.”
John Hutson, chief executive of JD Wetherspoon, added: "We are delighted to be opening The Beehive. JD Wetherspoon continues to enjoy great success with its pubs at Gatwick Airport and is proud to be associated with a thriving and successful airport.”
Wetherspoon currently operates four pubs at Gatwick airport – two in the North Terminal and two in the South Terminal.
Gatwick airport currently sees 32 million passengers through its doors every year.