The Edinburgh-based company will use the the Bank of Scotland investment to open its first English hotel outside of London – a 177-room site in Bath.
Though Apex first purchased the property in 2014, the hotel is not due to open until summer next year.
The £35m site will feature a swimming pool and gym and create 120 jobs.
Ian Springford, chairman at Apex Hotels, said: “The new hotel in Bath is an exciting opportunity for the group and we’re proud to have secured a great location in a booming city."
Though Apex did not comment on any future English expansion plans, Springford said the new site supported the company's 'long-term visions of growth'.
The hotel will be the tenth UK site for the group, which has an annual turnover of £52.7m.
Apex currently operates three hotels in London, four in Edinburgh, and one in both Glasgow and Dundee.
Alistair Macpherson, relationship director at Bank of Scotland, said: “We’re committed to supporting ambitious business like Apex Hotels as they expand into new markets, which will allow them to drive long-term growth.”