The venue, which has hosted live performances by established acts such as Oasis, Blur and Coldplay, becomes The Columbo Group's 10th site and follows its acquisition of the Jazz Café earlier this year.
The Columbo Group CEO Steve Ball said the Barfly would continue to operate in its present form, with plans to restore the iconic venue to its former glory at a later date.
Later this month the group will re-open the Jazz Café, the Camden nightclub it acquired in January. Structural, technical and aesthetic changes have been made to the site, which has hosted live performances by a number of soul, hip hop and jazz stars over the years.
The company will also launch its first music festival, Sunfall, in London's Brockwell Park on 9 July.
Camden's Barfly and The Jazz Café join The Old Queens Head in Islington, Paradise By Way Of Kensal Green, XOYO in Shoreditch, The Blues Kitchen in Camden, Shoreditch and Brixton, The Nest in Dalston and Phonox in Brixton in The Columbo Group's portfolio of restaurants, bars, nightclubs and live music venues.