The attraction received planning permission for a hotel in June 2015, but has submitted a new design following public concern about the visual impact and scale of the proposal.
The new-look project has been split in to two blocks and will use stone cladding and timber poles to blend in to the surrounding countryside.
A meadow and orchard will be planted around the hotel, and new classrooms will be used for the site’s apprenticeship and degree-level courses.
If the plans are approved construction will start in late 2017 with the aim of opening the hotel in April 2018.
Eden believes the hotel will help develop its wider estate beyond the world-famous Biomes.
More than 18m people have visited the project since it launched in 2001, contributing more than £1.7bn to the Cornish economy.
Eden executive director David Harland said: “We have always aspired to have a hotel at Eden and this is the culmination of years of work towards that aim.
“We feel that hotel accommodation at Eden would not only give an attractive option to our day visitors, but also increases Eden’s appeal as a venue for weddings, conferences and events.”
The hotel will have high standards of accessibility and feature an energy-efficient design.
“We are very pleased with this design and are grateful to our neighbours for their input into the plans, helping us ensure the building has minimal impact on the surrounding environment,” said Harland.
In 2014 Eden opened a 58-room on-site hotel in partnership with the Youth Hostel Association (YHA), aimed at school groups visiting the site.