The brand, which was bought by Cote in 2016, shut its Guildford restaurant on 28 March.
It was the first Jackson & Rye to open outside of London, but it lasted just 15 months.
The group also closed its site at Kingston’s Riverside scheme in January, and pulled out of opening in Birmingham in late 2017 after failing to reach a deal with the landlord.
The company is left with four London restaurants in Soho, Bank, Chiswick and Richmond.
A Twitter post confirming the most-recent closure said: “Our Guildford restaurant is now closed…we are very grateful for your ongoing support and patronage.”
Jackson & Rye, which serves a mix of French and American-inspired dishes, launched in 2013 with the backing of serial restaurateur Richard Caring.
It was sold to Cote alongside all-day group Limeyard for an undisclosed sum in January 2016.
Limeyard currently has three restaurants in Ealing, Staines and Leeds, while Cote also runs around 90 sites under its eponymous French café brand.