The 75-cover restaurant and taproom will open within Transport For London’s Wood Lane railway arches development, with table and bar seating available and a further 30 covers accessible on the terrace.
Interior designs will take some inspiration from American diner culture with exposed brick offset by Midwestern steel, industrial lighting fixtures, and retro Americana diner stools.
The menu will be focused on 'authentic' Kansas City-style barbecue, with meats cooked 'low and slow' in the restaurant’s smoker.
Options will include 16-hour oak smoked brisket; 14-hour applewood smoked pork; burgers; and meat samplers with brisket, pulled pork, sausage and slaw.
There will also be a selection of vegan and veggie dishes available.
Drinks will include a curated cocktail and wine list, as well as a rotating selection of London craft beers.
Prairie Fire was founded in 2013 by Kansas City native Michael Gratz, and previously traded at food markets and festivals across London.
The announcement comes little more than a week after it was confirmed that famed London barbecue restaurant Pitt Cue, which closed its doors back in June, is to return after being bought back by one of its original founders.