The Thai group opened in the Christopher Place shopping centre in 2016, but the restaurant shut its doors last month with all staff relocated to other sites.
It follows the closure of Busaba sites in Liverpool and Manchester in 2017 after the company is understood to have invested over £3m in the openings.
The chain also announced it had secured a site in Leeds in 2016, but it has yet to launch.
Busaba, launched in Soho by Wagamama founder Alan Yau in 1999, is now left with 13 sites in London.
It comes after Busaba Eathai Holdings Ltd, the group’s parent company, reported pre-tax losses of nearly £18m in the year to 27 May 2017.
The company said in a statement that it was focusing on “delivering operational excellence and memorable guest experiences” in its London estate going forward.
Busaba opened its first site outside the capital in Bicester Village in Oxfordshire in 2011, but it shut within two years.
A restaurant also launched under franchise in Dubai in 2015, but it is no longer trading.