The fresh pasta-focused brand, which is owned by Trullo founders Jordan Frieda and chef Tim Sidadatan, launched its first site in Borough Market in 2016.
Since opening, the restaurant has become synonymous with having queue times north of an hour during busy lunch and evening services.
In a short statement regarding the new opening, Padella said: “[We] will continue to serve simple and affordable pasta dishes and will be moving [our] existing bakery to the site.”
Frieda has previously said Padella was born out of a desire to make handmade fresh pasta accessible to all.
The pasta menu at the Borough Market restaurant ranges between £5 and £11 in price, and features dishes including tagliarini with anchovy butter and breadcrumbs; fettuccine with Cobble Lane Cured ‘nduja, mascarpone and lemon; and pappardelle with eight-hour Dexter beef shin ragu.
London’s market for accessibly-priced fresh pasta restaurants has grown significantly in recent years, with the likes of Pastaio, Bancone and Lina Stores all opening in the time since Padella first launched.
Last month, neighbourhood-style pizza brand Zia Lucia opened its first dedicated fresh pasta restaurant Berto next door to its pizzeria on Holloway Road.
And last weekend the team behind Franco Manca launched its new fresh pasta concept Strozzapreti inside KERB’s new Seven Dials Market in Covent Garden.